Journal: BMC Biotechnology
Article Title: Accurate non-invasive image-based cytotoxicity assays for cultured cells
doi: 10.1186/1472-6750-10-43
Figure Lengend Snippet: Comparison of the dose response plot of cisplatin after 72 h in the A2780R cells . Both plots display cell viability relative to non-treated cells vs -log [cisplatin]. (A) measure with the MTT assay and (B) measure with the CloneSelect Imager system. The pEC 50 value is denoted at the crossing of the solid lines. The concentration of cisplatin used (8.47 nM, 25.4 nM, 76.2 nM, 0.229 μM, 0.686 μM, 2.06 μM, 6.17 μM, 18.5 μM, 55.6 μM, and 167 μM) plotted as -log C vs cell viability. The error bars indicate the accuracy and reproducibility.
Article Snippet: These results demonstrate that the pEC 50 values obtained with the CloneSelect™ Imager system and the MTT assay are in close agreement.
Techniques: MTT Assay, Concentration Assay